Amylase Production by Thermotolerant Isolates of Bacillus licheniformis

dc.contributor.advisor
dc.contributor.author	Elham Sherief Dawood
dc.contributor.author	الهام شريف داود
dc.contributor.author	Ibrahim S. A.
dc.date.accessioned	2017-08-13T06:13:49Z
dc.date.available	2017-08-13T06:13:49Z
dc.date.issued	2011
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dc.identifier.uri	http://hdl.handle.net/123456789/1796
dc.description	ACT-Biotechnology Research Communications 2:1 (2012) 100-103	en_US
dc.description.abstract	Thermophilic processes appear more stable, rapid and less expensive and facilitate reactant activity and product recovery. Amylases have a quarter of the world enzyme market and thermostable amylases possess extensive commercial applications. Since little work has been done on strain isolation, growth and enzyme yield optimization, the level of thermophilic enzyme production remains relatively low. Therefore, large scale exploitation of thermophiles requires further intensive and integrated work. The present study describes isolation of four amylase producing bacilli from Sudanese soil. The isolates were identified and named as Bacillus licheniformis (SUDK1, SUDK2, SUDK4 and SUDO) . These isolates were tested for the production of amylase enzymes as they recorded the largest zone of activity. Amylase activity was determined using DNS method. The strain was cultured in liquid media to produce amylases. The enzyme production conditions of the newly isolated bacillii revealed that the maximum enzyme production after 24 h of cultivation at alkalophile pH and 50°C. 5m M Mg++ ions, 1% soluble starch in production medium enhanced the enzyme productivity for all isolates . 1% Peptone was the best nitrogen source for all isolates except SUDK2 which needed malt extract. The results showed that B. licheniformis (SUDK1, SUDK2, SUDK4 and SUDO) is a good producer of extracellular amylase at high temperatures which could be an indication that amylase produced would be thermostable and suitable for application in starch processing and food industries.	en_US
dc.subject	Bacillus licheniformis	en_US
dc.subject	thermo-stable amylase	en_US
dc.subject	culture conditions	en_US
dc.title	Amylase Production by Thermotolerant Isolates of Bacillus licheniformis	en_US
dc.type	Article	en_US