Abstract:
The study investigates Caprine Subclinical Mastitis (CSM) using local breed (Nubian), Saanen and Cross breed (Nubian+Saanen) goats in Atbara Area, Sudan. The study was carried out by using two field tests; California mastitis test (CMT) and White side test (WST). Results revealed that the prevalence of CSM out of 100 half/udder milk samples was 56% and 44% according to CMT and WST, respectively. Bacterial isolation and identification was done according to Cowan and Steel. The predominant isolated bacteria were: Staphylococcus, Pasteurella, Aeromonas, Micrococcus, Escherichia and Klebsiella. Streptococcus, Corynebacterim and Enterobacteria were isolated with lesser frequencies. Results revealed that Nubian breed was more resistant to causative bacteria (33.3% susceptibility), followed by Saanen (66.6%) and Cross breed (88.8%). Antimicrobial susceptibility tests were carried out by disc diffusion method on the Mueller Hinton agar. Out of six studied antibiotics Ciprofloxacin and Gentamicin showed 100% activity against both G+ve and G-ve isolates, while Chloramphenicol showed 100 and 60% activity against G+ve and G-ve, respectively. However 77.7% of isolates were resistant to Amoxicillin. CMT is more precise than WST in screening for CSM.